Proof-reading 3'5' exonucleases isolated from rat liver nuclei
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چکیده
منابع مشابه
The metabolism of isolated rat-liver nuclei.
The investigations described in this paper were undertaken as a preliminary to the study of the biochemical changes in the nucleus of the rat-liver cell during the induction of liver cancer. Allfrey (1954) and Stern & Mirsky (1953) described the isolation in aqueous media of nuclei from the thymus gland of the calf. Such preparations in vitro incorporated glycine and other amino acids into nucl...
متن کاملEffect of calcium and calmodulin on RNA synthesis in isolated nuclei from rat liver cells.
Calcium regulates a great variety of intracellular processes [l-4]. In muscular contraction [5] or the activation of adenylate [6,7], calcium, acting as a coupling or second messenger 141, binds to specific proteins like troponin [5] or calmodulin 16-81 to exert its action. In RNA synthesis, activation by calcium is not well understood. Calcium stimulates RNA synthesis in slices of ventricular ...
متن کاملDiphosphopyridine nucleotide pyrophosphorylase in the nuclei isolated from poisoned and regenerating rat liver.
Atkin, W. R. & Thompson, F. C. (1937). Procter'8 Leather Chemi8ts' Pocket Book, p. 136. London: E. and F. N. Spon Ltd. Barton, D. H. R. (1953). J. chem. Soc. p. 1027. Bate-Smith, E. C. & Swain, T. (1953). Chem. & Ind. p. 377. Chandorkar, K. R. & Kulkarni, A. B. (1957). Curr. Sci. 26, 354. Clark-Lewis, J. W. & Roux, D. G. (1959). J. chem. Soc. p. 1402. Evelyn, S. R. (1954). J. Soc. Leath. Tr. Ch...
متن کاملA novel 40S multi-snRNP complex isolated from rat liver nuclei.
Two structurally distinct RNP complexes (MI and MII), each with a sedimentation value of approx. 40S, were isolated from rat liver nuclear extracts by sucrose gradient centrifugation and subsequent native gel electrophoresis of the 40S hnRNP-containing fractions. MII RNP contained the bulk of hnRNA and hnRNP proteins (i.e. the 32-45KD core proteins and polypeptides of 60-80 and 110-130KD). MI R...
متن کاملTransmethylation and transguanylylation in 5'-RNA capping system isolated from rat liver nuclei.
Rat liver nuclei were isolated and sonicated for extraction in order to study the capping of RNA. The guanosine 7-methyltransferase was purified from the extract by hydroxylapatite column chromatography with stepwise addition of phosphate buffer. It was assayed by using as methyl acceptor synthetic G(5')ppp(5')G and S-adenosylmethionine as donor. The enzyme appeared in a sharp peak at 160 mM. T...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1993
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1993.tb18269.x